Significance of the collagen type Ⅳ in the auto-fluorescence diagnosis of cardiac cancer
- VernacularTitle:Ⅳ型胶原在贲门癌自体荧光诊断中的实验研究
- Author:
Wei LI
;
Shenglin CHEN
;
Yongming ZENG
;
Juntian CHEN
;
Yuwen GUO
;
Xinxin LI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Cardia;
Collagen type Ⅳ;
Collagen type Ⅲ;
Fluoroimmunoassay
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the distribution of collagen type Ⅲ and Ⅳ in mucosa of normal and carcinoma cardiac tissues and to analyze the influence of changed collagen on auto-fluorescence after canceration.Methods Using immunohistochemical technique with monoclonal anti-collagen typeⅢ and Ⅳ,the difference of distribution in extracellular matrix of two types collagen between normal and carcinoma tissues from 16 patients were measured with semi-quantitative score methods.Results In normal mucosa,the epithelial basement membrane showed an intense positive reaction for monoclonal anti-collagen Ⅳ and an intensively stained ring band was formed around the grand basement membrane.The collagen type Ⅲ formed ring-like structures apparently representative of fibriform collagen immediately adjacent to the epithelial basement membrane.In carcinomatous mucosa,immunoreaction of the carinomatous cell nest was negative,and only remnants of positively were seen at the site of the former membrane by collagen type Ⅳ.The change of collagen type Ⅲ was not apparent and the positive reaction in carcinomatous stroma was more intensity than in normal stroma.There was significant difference in distribution of collagen type Ⅲ and Ⅳ between the normal and carcinomatous tissues(P
