Study on apoptosis and changes of cell cycle in ovarian cells induced by paclitaxel
- VernacularTitle:紫杉醇诱导卵巢癌细胞凋亡及细胞周期改变的研究
- Author:
Zhiying YU
;
Liwen LI
;
Jing DU
;
Junxia XIA
;
Jun LUO
;
Qi LUO
- Publication Type:Journal Article
- Keywords:
Paclitaxel;
Ovarian neoplasms;
Apoptosis;
Cell cycle
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether paclitaxel can efficiently induce the apoptosis of ovarian cell HO-8910,and to study the relationship between the apoptosis of cells and the cell cycle.Methods With the treatment of paclitaxel with different concentrations and different time,the morphologic change of HO-8910 ovarian cells was observed using fluorescence microscopy and transmission electron microscopy(TEM),and the apoptosis of cells and the changes of cell cycle were determined by flow cytometry(FCM). Results The typical changes of HO-8910 cell apoptosis were observed by TEM and Fluorescence microscopy.With the treatment of paclitaxel,the HO-8910 ovarian cells were firstly arrested in G_2/M phase,and the typical ultrastructural changes of apoptosis were appeared only after the cells were apparently arrested in G_2/M phase.Conclusion Paclitaxel can induce the apoptosis of HO-8910 cells and the apoptosis is associated with the blockage of G_2/M phase in cell cycle.
