Construction of a VEGFR2 DNA Vaccine Against Tumor Angiogenesis
- VernacularTitle:抗肿瘤血管生成VEGFR2 DNA疫苗的构建
- Author:
Mingqing CHEN
- Publication Type:Journal Article
- Keywords:
VEGFR2;
Flk-1;
DNA vaccine;
Antineoplastic angiogenesis
- From:
Journal of Kunming Medical University
1989;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construet a novel DNA vaccine pcDNA3.1+/flk-1_((n1-4)) against tumor angiogenesis.Methods flk-1_((n1-4)) cDNA amplified by RT-PCR was inserted into eucaryotic expression vector pcDNA3.1+ and the recombinant plasmids pcDNA3.1+/flk-1_((n14)) was constructed.Confirmed by DNA sequence analysis,the recombinant plasmids was transfected into eucaryotic expression system COS-7 cells by lipofectamine.The protein expression of flk-1_((n1-4)) was detected by Western blot. Results Extracellular loop 1-4 of flk-1 was cloned and the recombinant plasmid was successfully constructed,which has been proved by DNA sequencing and compared with the data in Gene Bank.The protein expression of flk-1_((n1-4)) was detected in eucaryotic expression system COS-7 cells.Conclusions The DNA vaccine against tumor angiogenesis was successfully constructed,which found a basis for the further study on animal experiment and clinical application.
