The Heterogeneity Transplant Detection and Primary Culture of Lung Cancer in 1 Patient
- VernacularTitle:1例肺腺癌组织的原代培养及异种移植检测
- Author:
Fengcai YAN
- Publication Type:Journal Article
- Keywords:
Lung adenocarcinoma;
Cell culture;
Heterogeneity transplant;
Immunohistochemistry
- From:
Journal of Kunming Medical University
1989;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of our present study is to culture a new female lung adenocarcinoma cell line in Xuanwei region of Yunnan province,detect its proliferation dynamics and heterogeneity transplant and provide an ideal experimental model for the study of lung cancer.Methods The cell line was derived from a surgical specimen of lung cancer,and obtained lung adenocarcinoma cell clone by limiting dilution.Its biologic characteristics were explored by light microscope,determination of the doubling time and growth curve,inoculation the cells in mice as well as immunohistochemistry.Results Morphological study,proliferation dynamics and immersed growth demonstrated the cultured cells' malignant characteristics.The cell line produced 100% tumors after axillary transplantation into the mice provided by the Animal Centre of Kunming Medical College.The results of immunohistochemistry indicated the cells' adenocarcinoma characteristics.Conclusions According to a series of analysis of the cell line in vitro,the present study gave an important implication and some theory basis of following research on human lung adenocarcinoma,biological characteristics,cancerization,the mechanism of metastasis,and exploitation for new anticancer medicine et al.
