Evaluation of polymerase chain reaction (PCR) for direct detection of M.tuberculosis in AFB smear negative clinical specimens of tuberculosis patients
- Author:
Ho Minh Ly
- Publication Type:Journal Article
- Keywords:
M.tuberculosis, tuberculosis
- MeSH:
Polymerase Chain Reaction;
diagnosis;
Tuberculosis
- From:Journal of Medical Research
2003;23(3):15-20
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Polymerase chain reaction (PCR) was used for direct detection of M.tuberculosis in negative clinical specimens. This method was conducted on all tuberculous specimens in 1997-2001 at hospitals. PCR could detect 60/100 (60%) sputum samples, 35/41 (85%) bronchial washes, 60/80 (70%) cerebrospinal fluids and 19/27 (70%) pleural fluids. Overall sensitivity of PCR for these AFB was 69%. False positive presented in only 2 out of 60 specimens of control group, the specificity therefore was 97%. For culture positive specimens: 60/86 tuberculosis meningitis, 19/27 tuberculosis pleural fluids, 35/41 pulmonary tuberculosis. The sensitivity of PCR for detection of M.tuberculosis ranged 60-85%, the specificity 90-100% for smear negative clinical specimens of patients with pulmonary tuberculosis and extra pulmonary tuberculosis.
