A preliminary study of stent grafts seeded by CD34~+ stem cells in venous system
- VernacularTitle:骨髓CD34~+细胞种植的覆膜支架应用于静脉系统的初步研究
- Author:
Wei LI
;
Xiaoming ZHANG
;
Jiyan XIE
;
Fen CHANG
- Publication Type:Journal Article
- Keywords:
Stents;
Antigens, CD34;
Bone marrow;
Seeding;
Endothelial cells
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CD34+ stem cells seeded stent graft in venous system. Method Twelve mongrel dogs were randomized into 2 groups. Stent grafts covered with PTFE or Dacron were implanted into infrarenal vena cava through a delivery system. In each group, 4 stent grafts were seeded by autogenous bone marrow CD34+ cells, and 2 treated by autogenous blood only as control. The grafts were explanted at 2 weeks. Light and electron microscopy were used to examine endothelialization of neointima. CD34 and Ⅷ factor stain were applied to identify endothelial cells. Results All stent grafts were patent except for one Dacron stent without seeding. Light, electron microscopy, CD34 and Ⅷ factor stain demonstrated that confluent endothelial cells appeared on the neointima of seeded grafts. No endothelial cells were detected in the non-seeding grafts. Conclusions Rapid endothelialization of stent grafts can be achieved through bone marrow CD34+ cells seeding in venous systems.