HBx facilitates VEGF expression in transplanted human hepatoma tissue of the nude mouse
- VernacularTitle:乙型肝炎病毒x蛋白促进裸鼠人肝癌模型的VEGF表达
- Author:
Donghua LI
;
Xiaoping CHEN
;
Wanguang ZHANG
;
Fazu QIU
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Hepatitis B virus;
Endothelial growth factors;
Proliferation;
Nude mouse
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism by which HBx facilitates the proliferation of hepatoma cells. Methods The expression of plasmid pHA-HBx encoding full length of HBx was transfected into HepG2 cell lines and the transformed cells were identified by RT-PCR. The nude mouse model of transplanted human hepatoma HBx-transfected HepG2 cells was established. The expression of VEGF both in HepG2 cell-formed tumors and HBx-transfected cell-formed tumors in nude mice was examined immunohistochemically. Results RT-PCR showed that HBx gene was integrated into the genome DNA of HepG2 cells and amplified; The rate of tumor formation in nude mice both of HepG2 cells and HBxtransfected HepG2 cells was 100%; The growth speed of HBx-transfected tumors was much faster than that in control group; The average volume of HBx-transfected tumors and non-transfected tumors was 2. 86?0. 34 cm3 and 2.48?0.22 cm3 respectively after 8 weeks(t=1.905 ,P
