Role of peroxisome proliferator-activated receptor ? in multidrug resistance of human hepatocellular carcinoma
- VernacularTitle:脂质过氧化物酶体增殖物激活受体?在人肝癌多药耐药中的作用
- Author:
Qi WANG
;
Xiaoping CHEN
;
Jian GUAN
;
Wanguang ZHANG
;
Bixiang ZHANG
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Gene expression;
Peroxisome proliferator-activated receptor ?;
Multidrug resistance
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of PPARa gene in human hepatocellular carcinoma. Methods Transmission elctron microscopy was used to study the morphologic differences between the MDR cell line HepG2/ADM and its mother cell line HepG2;Real-time RT-PCR and Western blotting were used to check the expression of PPARa in mRNA level and protein level in the two cell lines respectively. Results Transmission elctron microscopy showed that the HepG2/ADM cells had more viliformed maculas on their nuclear memberanes, more vacuoles in their cytoplasmas, more rough endoplastic reticulums; the PPARa gene was down regulated in the HepG2/ADM cells. Conclusion PPARa is related with the multidrug resistance phenomenon in the HepG2/ADM cells. The down regulation of PPARa may be one of the mechanisms conferring the forming of MDR in tumor cells.
