Effect of perforin and granzyme B in peripheral blood lymphocytes on diagnosis of acute rejection in renal transplantation
- VernacularTitle:外周血淋巴细胞穿孔素和颗粒酶B在诊断移植肾急性排斥反应中的应用
- Author:
Jiangping CHANG
;
Feng WANG
;
Ming SHI
;
Xintao ZHANG
- Publication Type:Journal Article
- Keywords:
Renal transplantation;
Acute rejection;
Diagnosis;
Perforin;
Granzyme B
- From:
Chinese Journal of Postgraduates of Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical value of perforin and granzyme B expression in peripheral blood lymphocytes and diagnosis of acute rejection in renal transplantation. Methods Sixty-seven recipients of renal allograft were included in the study. The expression of perforin and granzyme B of peripheral blood lymphocytes was studied by quantitative reverse transcription polymerase chain reaction (RT-PCR). The recipients were divided into four groups, including 7 cases of acute rejection as group 1, 8 cases of delayed graft function as group 2, 27 cases of stable function as group 3, 25 cases of long-term survival as group 4. Results The expression of perforin and granzyme B in group 1 was significantly higher than that of the other three groups (P
