Oridonin enhances phagocytosis of apoptotic U937 cells by macrophage-like cells
- VernacularTitle:冬凌草甲素增强巨噬细胞对凋亡的U937细胞的吞噬作用
- Author:
Yanqiu LIU
;
Song YOU
;
Chunling ZHANG
;
Shinichi TASHIRO
;
Satoshi ONODERA
;
Takashi IKEJIMA
- Publication Type:Journal Article
- Keywords:
Oridonin;
U937 cells;
Macrophages;
Apoptosis;
Phagocytosis
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of oridonin on the phagocytosis of apoptotic U937 cells by macrophage-like cells. METHODS: DNA agarose gel electrophoresis, Giemsa staining, Hoechst 33258 staining and photomicroscopical observation were used. RESULTS: UV irradiation (2.4 J/cm~2, 4 min) induced U937 cell apoptosis. Marked DNA fragmentation in agarose gel electrophoresis was observed. Oridonin augmented phagocytosis of apoptotic U937 cells by U937 cell-derived macrophages in a time- and dose-dependent manner. However, less effect on synthetic fluoresbrite micropheres was observed. The oridonin-augmented phagocytosis was attenuated by anti-human TNF? or anti-human IL-1? antiserum. In addition, the similar effect of phagocytosis was observed in oridonin-treated human monocyte-derived macrophages at 4 day maturation. CONCLUSION: Oridonin enhances phagocytosis of apoptotic U937 cells by macrophage-like cells. The releases of TNF? and IL-1? are involved in this mechanism.
