Burn rat serum suppresses transient outward potassium current (I_(to))
- VernacularTitle:烧伤大鼠血清抑制瞬间外向钾电流
- Author:
Jie LIU
;
Jianxin DENG
;
Qiaobing HUANG
;
Xuliang HUANG
;
Kesen ZHAO
- Publication Type:Journal Article
- Keywords:
Transient outward potassium current;
Transfection;
Heart;
Burns
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study I_ to channel function in severe burn and its contribution to cardiac dysfunction induced by severe burn. METHODS: CHO-K1 cells were transfected with human Kv4.3, the major subunit of human I_ to channel. The expressed Kv4.3 channels were recorded by whole-cell patch clamp and the effects of rat burn serum on Kv4.3 current densities and kinetics were observed. RESULTS: Kv4.3 channels expressed in CHO-K1 cells were endowed with the characterization of fast activation and inactivation, which was quite similar to that of native I_ to channels in cardiomyocytes. Rat's burn serum at the concentration of 2% decreased the current density significantly. At +40 mV, the current density in control group was (67.6?15.1) pA/pF, in contrast to (32.3?9.7)pA/pF in burn serum-treated group (P
