Effects of the antibodies against ?_1-adrenergic receptor on the proliferation of vascular smooth muscle cells in rats
- VernacularTitle:抗?_1-肾上腺素能受体抗体对培养大鼠主动脉平滑肌细胞增殖的影响
- Author:
Zihua ZHOU
;
Yuhua LIAO
;
Bin WANG
;
Liudong LI
;
Fen WEI
;
Min WANG
- Publication Type:Journal Article
- Keywords:
Antibodies;
Receptors, adrenergic, alpha;
Cell proliferation;
Muscle, smooth, vascular;
Protein c-jun;
Protein c-fos
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The autoantibodies against ?_1-adrenergic receptor that was found in patients with malignant hypertension, primary hypertension and refractory hypertension has the agonist activity liked the NE, and may play a role in hypertension. In this paper, the effects of this antibody on vascular smooth muscle cell (VSMC) proliferation and its mechanism were to be studied. METHODS: The cultured rat VSMC proliferation induced by the antibodies against ?_1- adrenergic receptor that was purified by the immune affinity chromatography, was measured by the BrdU cell proliferation assay and cell cycle distribution. The expression of c-jun and c-fos were determined by RT-PCR and Western blotting. RESULTS: Compared to the normal IgG, the antibodies against ?_1-adrenergic receptor promoted the VSMC proliferation and increased the mRNA and protein expression of the c-jun significantly. The role was similar to the norepinephrine, and all was blocked by prazosin, while the mRNA and protein expression of c-fos were not affected by the antibodies. CONCLUSION: The antibodies against ?_1-adrenergic receptor promote the rat VSMC proliferation, and increase the expression of c-jun, which maybe play a role in the vascular remodeling in hypertension.
