Hypoxia induces alteration of MMP-2 and TIMP-1 expression in pulmonary artery fibroblasts
- VernacularTitle:缺氧对肺动脉成纤维细胞MMP-2、TIMP-1表达的影响
- Author:
Wanli MA
;
Hong YE
;
Jianbao XIN
;
Ming BAI
- Publication Type:Journal Article
- Keywords:
Anoxia;
Pulmonary artery;
Fibroblasts;
Matrix metalloproteinases;
Tissue-inhibitor of metalloproteinase-1
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of hypoxia on the expression of MMP-2 and TIMP-1 in pulmonary artery fibroblasts. METHODS: MMP-2 activity was measured by using gelatin zymography. TIMP-1 protein level was determined by immunocytochemistry and Western blotting. mRNA expression was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: The production of MMP-2 mRNA and protein was down-regulated in pulmonary artery fibroblasts exposed to hypoxia for 24 h. Gelatin zymography also demonstrated that lower level of MMP-2 activity was induced after hypoxia. Meanwhile, hypoxia induced the up-regulation of TIMP-1 mRNA and protein in pulmonary artery fibroblasts. CONCLUSION: Hypoxia induces unbalance of MMP-2/TIMP-1 in pulmonary artery fibroblasts, which may contribute to the mechanism of hypoxic pulmonary vascular remodeling.
