Effect of glomerular intercellular interaction under high glucose concentration on production of ROS and TGF-?_1 in co-cultured ECV304 cells and intervention with tea polyphenols
- VernacularTitle:高糖时细胞间相互作用对共培养ECV304细胞的影响及茶多酚的干预作用
- Author:
Hong DENG
;
Hailang LI
;
Kai WANG
;
Yu ZENG
;
Bicheng LIU
- Publication Type:Journal Article
- Keywords:
Coculture;
Glucose;
Tea polyphenols;
Reactive oxygen species;
Transforming growth factor beta;
ECV304 cells
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of glomerular intercellular interaction under high glucose concentration on the production of reactive oxygen species (ROS) and transforming growth factor ?_1 (TGF-?_1) in co-cultured human ECV304 cells, and to study the intervention with tea polyphenols (TPs). METHODS: The endothelial cells were cultured alone or co-cultured with mesangial cells in high glucose media with or without TPs for 0 h, 12 h and 36 h, respectively. The activity of SOD and the content of MDA in the media of the system were detected by spectrophotometry. The expression of TGF-?_1 mRNA in the endothelial cells was measured by using semi-quantitative reverse transcription PCR (RT-PCR). RESULTS: High glucose decreased the activity of SOD, increased the content of MDA and up-regulated the expression of TGF-?_1 mRNA in co-cultured ECV304 cells and the effect became more prominent than the single-cultured cells. TPs interrupted it more effectively. CONCLUSION: These data suggest that there is interaction between mesangial cells and ECV304 cells under high glucose concentration. The interaction may markedly up-regulate the production of ROS and the expression of TGF-?_1 in co-cultured ECV304 cells. TPs may protect ECV304 cells by intervening intercellular interaction.
