Effect of ischemic preconditioning on contents of cytochrome C and mitochondrial calcium in rats after focal cerebral ischemic-reperfusion
- VernacularTitle:缺血预处理对大鼠缺血性脑损伤线粒体钙、细胞色素C水平的影响
- Author:
Shuqiu WANG
;
Apeng CONG
;
Yongyi LI
;
Chunfang FU
;
Jianhua ZHANG
;
Shuxiang WANG
;
Yazhen LU
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Mitochondria;
Cytochrome C;
Calcium
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of ischemic preconditioning on contents of cytochrome C and mitochondrial calcium in rats after focal cerebral ischemic-reperfusion. METHODS: Focal cerebral ischemic model was made by occlusion of right middle artery in Wistar rats (ischemia for 2 h and reperfusion for 4 h). Rats were randomly divided into three groups: ischemia pretreatment, model and sham operation. Rats in ischemic pretreatment group were undergone transient ischemic preconditioning (30 min) and reperfusion (72 h). The contents of cytochrome C were measured according to Zhangjuntian's improved methods. The contents of mitochondrial calcium were detected by flame atom absorption. RESULTS: The contents of mitochondrial cytochrome C and calcium in model group were significantly lower than those in sham operation (P
