Crucial role for ecto-5'-nucleotidase (CD73) in invasion and migration of human breast cancer cell lines
- VernacularTitle:CD73对人乳腺癌细胞侵袭力和运动力的影响
- Author:
Tingting ZHOU
;
Ping ZHOU
;
Lianhua YIN
- Publication Type:Journal Article
- Keywords:
5'-nucleotidase;
Adenosine;
Breast neoplasms;
Neoplasm invasiveness;
Cell movement
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To understand the expression of ecto-5'-nucleotidase (CD73) and explore its effect on the motility and invasion ability of breast cancer cell lines. METHODS: ① CD73 mRNA expression was analyzed by semiquantitative RT-PCR. Surface CD73 activity in breast carcinoma cell lines was measured by HPLC according to the conversion of E-AMP to E-ADO. ② Plasmid pcDNA-NT5E was transfected into human low metastatic breast cancer cell line T47D with lipofectinamine2000. CD73 mRNA expression was detected by semiquantitative RT-PCR and surface CD73 activity was determined by HPLC before and after transfection. The effect of pcDNA-NT5E transfection on motility and invasion ability was observed by in vitro invasion assay. RESULTS: ① The RNA expression and enzyme activity of CD73 were variable in different breast cancer cell lines. ② The transfect-T47D overexpressed CD73 at mRNA level and with high enzyme activity. Constitutively active CD73 drove cells transmembrane and invasion into matrigel. Promotion effects were statistically significant (P
