IFN-? reinforces the inhibitory effect of tumor necrosis factor-related apoptosis-inducing ligand on apoptosis of human colon cancer cell line RKO cells
- VernacularTitle:?-干扰素加强TRAIL抑制人大肠癌细胞株作用的研究
- Author:
Chao HE
;
Wenxian HU
;
Xiaotong HU
;
Xuefeng HUANG
- Publication Type:Journal Article
- Keywords:
Interferon type Ⅱ;
RKO cell line;
Tumor necrosis factor-related apoptosis-inducing ligand
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the influence of IFN-? on the role of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in inducing apoptosis of RKO cell line. METHODS: Survival fraction and apoptosis were measured by MTT method and flow cytometry (FACS). RESULTS: Survival fraction of IFN-? group, TRAIL and IFN-? 72 h+TRAIL group were 99.28%, 85.45%, 52.60%, respectively. The percentage of apoptotic cells of IFN-? group, TRAIL group, IFN-? 24 h+TRAIL group, IFN-? 48 h+TRAIL and IFN-? 72 h+TRAIL group were 1.51%, 2.38%, 4.97%, 13.30%, 21.00%, respectively. The percentage of apoptotic cells of IFN-? 24 h+TRAIL group was higher than the sum of IFN-? group and TRAIL group (P
