The mRNA expression of CC chemokines in peripheral blood mononuclear cells from patients with chronic urticaria
- VernacularTitle:慢性荨麻疹患者外周血单一核细胞CC型趋化因子的表达
- Author:
Hui TANG
;
Jinhua XU
;
Zhizhong ZHENG
- Publication Type:Journal Article
- Keywords:
Urticaria;
Chemokines, CC;
Leukocytes, mononuclear
- From:
Chinese Journal of Dermatology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mRNA expression of CC Chemokines, including RANTES (regulated upon activation, normal T cell expressed and secreted), monocyte chemoattracctant protein-1 (MCP-1), monocyte chemoattracctant protein-3 (MCP-3) and macrophage inhibitory protein 1? (MIP-1?), in peripheral blood mononuclear cells (PBMCs) of patients with chronic urticaria, their mutual correlation, and the relationship between the expression level of CC chemokines and the serum level of IgE and eosinophil cationic protein (ECP) in the patients. Methods Reverse transcription-polymerase reaction (RT-PCR) was applied to semiquantitatively analyze the mRNA expression of RANTES, MCP-1, MCP-3 and MIP-1? in PBMCs from 31 patients with chronic urticaria and 22 healthy subjects. Results The mRNA expression of RANTES, MCP-1, MCP-3 and MIP-1?was significantly higher in the patients than those in the normal controls (P 0.05). In the patients, the mRNA expression of RANTES negatively correlated with the serum level of IgE and ECP (P
