The effects of L-NAME on invasion and metastasis of human colorectal carcinoma cell line
- VernacularTitle:L-NAME对大肠癌细胞侵袭和转移的影响
- Author:
Libo YU
;
Wenzhou SUN
;
Xinshu DONG
;
Haitao XU
- Publication Type:Journal Article
- Keywords:
Colorectal Neoplasms/pathol;
Neoplasms Metastasis;
L-NAME/pharm
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of L-NAME on invasion and metastasis of human colorectal (carcinoma) cell line(LS-174T) and explore its possible mechanism.Methods The LS-174T cells were co-incubated with L-NAME at various concertrations.Griess techniqe was used to examine the effect of(L-NAME) on excretion of nitric oxide(NO) of the cells.The effects of L-NAME on invasion and migration of LS-174T were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted(basement) membrane.Expression of MMP2 mRNA and TIMP2mRNA in LS-174T cells was measured by(RT-PCR).Results(1)L-NAME decreased the excretion of NO of the cells in a dose-dependent manner.(2)The ability of the L-NAME treated LS-174T cells to invade the reconstituted basement membrane(increased) significantly with the concentration and time,at the concentration of 0.2 mmol/L,0.4 mmol/L,0.8mmol/L and 1.0mmol/L,respectively,after 72 hour,the inhibition rates were 10.29%,19.62%,34.08% and 42.23%,respetively.(P
