Experimental study on inhibition of cell growth in colon cancer cell lines by non-steroidal anti-flammatory drugs
- VernacularTitle:非甾体类消炎药抑制结肠癌细胞株细胞生长的实验研究
- Author:
Xuejun LAO
;
Minrong CAO
;
Haiping JIANG
;
Hanlin TANG
;
Zhongping LIANG
;
Zhao PANG
- Publication Type:Journal Article
- Keywords:
Colon Neoplasms/durg ther;
Anti-Inflammatory Agents,Non-Steroidal/theruse;
Tumor Cells,Cultured/drug eff;
Anti-Inflammatory Agents,Non-Steroidal/pham
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect and mechanism of non-steroidal anti-flammatory drags(NSAIDs) on the colon cancer cell growth by using S-nitrosoglutathione(GSNO) which can produce nitric oxide.(Methods) Apoptosis of 3 colon cell lines were evaluated by cell growth curve and flow cytometry,the PGE_2 levels in cell culture supernatants were determined by competitive enzyme immunoassay method,and the(protein) expression of COX-1 and COX-2 were analyzed by Western blot.Results The production level of PGE_2 was increased with CSNO treated concentration and time.Using 500?mol/L CSNO treatment for 48h,the expression level of COX-1 and COX-2 protein increased.NASIDs can block the production of PGE_2 but had no effect on the inhibition of cell growth induced by GSNO.Conclusions GSNO can increase PGE_2(production) and induce COX-1 and COX-2 protein expression in a dose-and time-dependent manner.Higher concentrations of GSNO also can inhibite cell growth and induced apoptosis in all 3 cell lines.NSAIDs can block production of PEG2 but NSAIDs are no effect on cell growth.
