A modified model of orthotopic small intestinal transplantation in rats
- VernacularTitle:大鼠原位小肠移植模型的建立与改进
- Author:
Lin FAN
;
Xiangming CHE
;
Wei ZHAO
;
Shufeng WANG
;
Ruyuan ZHANG
;
Li ZHANG
;
Fen ZHANG
- Publication Type:Journal Article
- Keywords:
Intestinal Transplantation;
Disease Models,Animal
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and stable model of orthotopic small intestinal transplantation(OIT) in rats.Methods The harvested en bloc segmental intestine consisted of donor abdominal aorta with superior mesenteric artery and portal vein.After in situ infusion with lactated Ringer′s solution,the graft was stored in 4℃ lactated Ringer′s solution.The segmental intestine transplantation was performed by end-to-side anastomosis of donor abdominal aorta to recipient abdominal aorta.The donor portal vein was anastomosed to the recipient left renal vein by "cuff anastomosis".The graft was anastomosed orthotopically by continuous(sutures).Results Sixteen intestine transplantations were,performed the average time for the arterial and(venous) anastomosis was 25?5 min and 4?1 min,respectively.Among the 16 recipient rats,13 survived more than 5 days.The average survival time was 10.35?2.84 days;the longest survival time was 21 days.Conclusions Graft harvesting,the technique of vascular and intestinal anastomosis and the(maintenance) of adequate blood volume are the key points for success of the operation.This successfully(established) model can serve as an excellent animal model for basic research of small intestinal trasplantation.
