Concentration Determination of m-Nisoldipine in the Plasma of Rhesus Monkeys by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定恒河猴血浆中间尼索地平的浓度
- Author:
Zhiqing ZHANG
;
Yongli WANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
m-Nisodipine;
Rhesus monkeys;
Plasma;
Concentration
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a RP-HPLC method for concentration determination of m-nisoldipine (m-Nis) in the plasma of rhesus monkeys.METHODS: The analysis was performed on a C18 column at 30℃ with a mobile phase of methanol-water (63∶37) at a flow rate of 1.0ml/min.The detection wavelength was 236nm.The sensitivity was 0.05AUFS and the internal standard was nimoldipine.RESULTS: The detectable concentration of m-Nis showed good linearity in the range of 1.75～ 448?g/L.The average recovery of extraction was 84.1%(RSD=4.09%), and the average recovery of method was 98.7%(RSD=2.76%).The processed plasma samples remained stable in quality after having been stored for 7 days, and the plasma remained stable in quality through repeated freeze thawing or stored at low temperature.CONCLUSION: The present method is highly sensitive and reproducible, as well as convenient, which can be used for concentration determination of m-Nis in the plasma of rhesus monkeys.
