Content Determination of Gatifloxacin in Human Plasma by HPLC
- VernacularTitle:高效液相色谱法测定人血浆中加替沙星浓度
- Author:
Qi PEI
;
Shikun LIU
;
Zuojun LI
;
Yulu ZHOU
- Publication Type:Journal Article
- Keywords:
Ggatifloxacin;
HPLC;
Plasma drug concentration
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC method for content determination of gatifloxacin in human plasma.METHODS: The Analytical column was C18,the mobile phase consisted of acetonitrile-30mmol/L ammonium acetate-triethylamine-orthophosphoric acid (20∶80∶1.0∶0.7) with a flow rate of 1.0ml/min,the detection was performed at UV 294nm.RESULTS: The calibration curve was linear in the concentrations ranging from 0.1 to10.0?g /ml (r=0.9 992).The detection limit was 0.05?g/ml.The intraday RSD was less than 8% and interday RSD was less than 10%.The average recovery was (101.67?3.06)%.CONCLUSION: The method is simple, sensitive, accurate and suitable for determination of gatifloxacin in human plasma and pharmacokinetic study.
