Determination of Minocycline in Human Plasma by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定人血浆中二甲胺四环素浓度
- Author:
Qing DAI
;
Yongchuan CHEN
;
Songqing LIU
;
Peiyuan XIA
;
Juying HE
- Publication Type:Journal Article
- Keywords:
Minocycline;
HPLC;
Drug concentration in plasma
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To develop a RP-HPLC method for determination of minocycline concentration in human plasma.METHODS:Chromatographic separation has been achieved on C18 column with acetonitrile-H2O-TFA(15∶85∶0.1)as the mobile phase, and oxytetracycline as internal standard.The detection wavelength was 350nm.The minocycline was extracted from buffered plasma(pH=6.5)by ethyl- acetate, and quantified by the ratio of minocycline peak area to that of internal standard.RESULTS :The linear range of minocycline detection concentration was 0.05～8?g/ml(r=0.9 999).The minimum detection concentration was 0.02?g/ml with an average recovery of 101.89% .The inter and intra-day RSD were both less than 5%.CONCLUSION :The present method is simple, rapid and accurate for determination of minocycline in human plasma.
