Effect of tetramethylpyrazine on expression of caspase-1 mRNA in the hippocampus of the developing rats following recurrent seizures
- VernacularTitle:川芎嗪对发育期大鼠惊厥性脑损伤后海马caspase-1 mRNA表达的影响
- Author:
Liqun LIU
;
Dingan MAO
;
Tao BO
;
Haitao BAI
;
Jie XIONG
- Publication Type:Journal Article
- Keywords:
caspase1;
brain injury;
brain development;
hippocampus;
seizure;
mRNA expression;
tetramethylpyrazine
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of caspase1 mRNA in hippocampus of(developing) rats following recurrent seizures and the effects of tetramethylpyrazine on it.Methods: One(hundred) and(sixty)two postnatal SpragueDawley rats of 20day old were randomly divided into three groups: the control group,the seizure group and the tetramethylpyrazine treated group.Seizure model of rats were induced by(inhalant) flurothyl daily in 6 consecutive days.Brain tissue was sampled at 6 hours,1 day,(3 days) and 7 days in each group after last seizure,and the expression of caspase1 mRNA in the hippocampus was(detected) by(reverse) transcriptionpolymerase chain reaction(RTPCR).At the same time,the water content was(detected) and the pathological changes in the hippocampus of rats were observed after recurrent seizures and the brain injury was evaluated by using a semiquantitative neuropathological scoring system.Results: In(tetramethylpyrazine) treated group,the levels of caspase1 mRNA in the hippocampus,water content of brain tissues and neuropathological score at different time points were obviously lower than those in seizure group(except water content of brain tissues at 7 days,all P
