Application and comparison of some methods of extraction DNA from peripheral blood
- Author:
Le Hoan
- Publication Type:Journal Article
- Keywords:
DNA, peripheral blood
- MeSH:
DNA;
blood;
Methods
- From:Journal of Medical Research
2003;23(3):3-6
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
DNA extraction is basis and important step in molecular biology technical. DNA was extracted from peripheral blood by some technique as: Perchlorate sodium, Acetate amonium. QIA amp minikit (Micro technique). All three methods obeyed these steps: destruction of red blood cells, destruction of white blood cells, destruction of protein. Among the techniques mentioned above, perchlorate sodium DNA extraction is the best technique, although it needs only a very small amount of blood (200 microlit) but technique’s price is high. The results indicated: DNA extraction by Perchlorate sodium was the most appropriate with the designed standard. Now, this technique is daily used at Bio-Medical Centre Laboratory, Hanoi Medical University.
