The effect of pingyangmycin on vascular endothelial cells cultured in vitro
- VernacularTitle:平阳霉素对体外培养血管内皮细胞的作用
- Author:
Ping LI
;
Shuimiao ZHOU
;
Hongliang ZHENG
;
Wu WEN
;
Zhaoji LI
;
Xiaohua SHEN
;
Junhui WEN
- Publication Type:Journal Article
- Keywords:
Antibiotics,Antineoplastic;
Endothelium Vascular;
Apoptosis;
Cell Cycle;
Cysteine Endopeptidases;
Hemangioma,Cavernous;
Head and Neck Neoplasms
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the inhibiting effect of Pingyangmycin on cultured vein endothelial cells in vitro and illustrate the mechanism of Pingyangmycin on treatment cavernous hemangioma of head and neck. METHODS By MTT assay,the inhibiting rates of cultured Ecv-304 cells which were managed with Pingyangmycin for 24h and 48h were compared respectively. The changes in the cell cycle,apoptosis,and Caspase 3 protein expression of the cells managed with Pingyangcin for 24h were examined by flow cytometry(FCM). RESULTS The results showed that the inhibiting rate of Ecv-304 was dependent on the concentration of Pingyangmycin. However,in the same concentration,the inhibiting effect for 24h was stronger than that for 48h. The percentage of G1 phase cells increased while the S phase decreased,but the percentage of G2 phase cells remained unchanged after managed with Pingyangcin for 24h. An apoptosis peak appeared before G1 phase. The apoptosis rate was also concentration-dependant. The expression of caspase 3 increased in every group of different concentration. CONCLUSION Pingyangmycin can possibly arrest the growth of Ecv-304 in the G1 phase through the mechanism of inducing apoptosis. Caspase 3 may play an important role in the induced apoptosis.
