The modified method for human nasal septum chondrocyte culture and the study of biological characters of the chondrocyte
- VernacularTitle:人鼻中隔软骨细胞培养法的改良及其生物学特性观察
- Author:
You CHENG
;
Qiuping WANG
;
Jinghong LI
;
Jiang DU
;
He QIN
;
Qiao TANG
;
Jinzhong HUANG
- Publication Type:Journal Article
- Keywords:
Nasal Septum;
Chondrocytes;
Cell Culture
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To established a viable and convenient method for human nasoseptal chondrocyte culture in vitro and study the biological characters of the chondrocyte. METHODS The chondrocytes were obtained by incomplete enzyme digestion of cartilage and were seeded with small piece of cartilage in 25 ml culture flasks coated with polylysine previously. This study determines the biologic synthesis of the chondrocytes by toluidine blue and type Ⅱcollagen immunohistochemistry reaction. The proteoglycan synthesis efficiencies of human nasoseptal chondrocytes cultured in vitro were measured by using 35S-Na2SO4 incorporation. The modified method and the conventional method in proteoglycan synthesis were also compared. RESULTS The results suggested that human nasoseptal chondrocytes cultured by modified method still show the normal structure and morphology, and maintain the function of metabolism and self duplication. With the modified method, we can get a higher cell survival rate and more cells. CONCLUSION The modified method is a viable and convenient method.
