Damage of the retrograde axial flow of retinal ganglion cells in diabetic rats at the early stage
- VernacularTitle:早期糖尿病大鼠视网膜神经节细胞逆行轴浆流损害的研究
- Author:
Yaowu QIN
;
Gezhi XU
;
Wenji WANG
- Publication Type:Journal Article
- Keywords:
Diabetes/physiopathology;
Retinal ganglion cell;
Staining and labeling
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether the retrograde axial flow of retinal ganglion cells (RGC) in diabetic rats at the early stage was damaged. Methods Diabetic model was induced by streptozotocin in 6 adult male Sprague-Dawley (SD) rats. Fluorogold (FG) was injected to the superior colliculi 4 weeks later. Streched preparation of retina was made 12 and 72 hours after the injection, and was stained after photographed by fluorescent microscope. The proportion of RGC with different sizes labeled by FG was calculated. Other 6 normal adult male SD rats were in the control group. Results Twelve hours after injection with FG, there was no difference of the total number of RGC in experimental and control group, but the ratio of small RGC was lower in experimental group than that in the control group; 72 hours after injection with FG, The number of RGC, especially the small RGC, decreased obviously in experimental group compared with the control group. Conclusion The speed of the retrograde axial flow of RGC in diabetic rats at the early stage is affected, and the small RGC are damageable.
