Effects of M?ller cells on expression of occludin in retinal vascular endothelial cells
- VernacularTitle:M?ller细胞对视网膜血管内皮细胞紧密连接蛋白表达的影响
- Author:
Hui YE
;
Gezhi XU
;
Wenji WANG
- Publication Type:Journal Article
- Keywords:
Glycosylation end proclucts, advanced;
M?ller cells;
Endothelium, vascular;
Occludin;
Blood-retinal barrier
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Obiective To investigate the change of the activity of proliferation in cultivated M?ller cells treated by advanced glycation endoproducts (AGEs), and the effect of these changes on expression of occludin in bovine retinal vascular endothelial cells (BREC). Methods The cultivated M?ller cells were devided into normal growth group and cultured with AGEs group. The cultured BREC were devided into 4 groups:group 1, without any medium; group 2, with normal growth M?ller cell medium (MCM); group 3,MCM treated by AGEs; group 4, without cell as the control. Enzyme-linked immuno sorbent assay was used to detect the content of occludin in the medium in the 4 groups. Results The content of expression of occludin was the most in group 2, less in group 1, and the least in group 3. Conclusion AGEs may promote the abnormal proliferation of M?ller cells and inhibit the expression of occludin in BREC.
