The effect of nerve growth factor on in vitro invasion of human cholangiocarcinoma cells
- VernacularTitle:神经生长因子对人胆管癌细胞体外侵袭力影响的研究
- Author:
Wei GUO
;
Shengquan ZOU
- Publication Type:Journal Article
- Keywords:
Cholangiocarcinoma;
Nerve growth factor;
Lymphocytes, tumor-infiltrating
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate if cancer cell-derived nerve growth factor participates in regulation of human cholangiocarcinoma cell invasion in vitro. Method After construction of a full-length human p-NGF expression vector, human cholangiocacinoma cell lines QBC939 was transfected with pcDNA3. 0-NGF. Western blot assays were used to detect the expression of ?-NGF. In vitro invasion of transfected cells was analyzed by Transwell assays. Result p-NGF was expressed by transfected QBC939 cells, compared with controls, the differences were significant (P
