Protection of hepatic ischemia-reperfusion injury by human IL-10 gene transfection in rats
- VernacularTitle:人白介素-10腺病毒转染对大鼠肝缺血再灌注损伤的保护作用
- Author:
Chen LIU
;
Mengchao WU
;
Baihe ZHANG
;
Xinghua WANG
;
Li HAO
;
Yixuan LIU
;
Zhenfu CUI
;
Qijun QIAN
- Publication Type:Journal Article
- Keywords:
Liver;
Reperfusion injury;
Interleukin-10;
Gene therapy;
Apoptosis
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protection against hepatic ischemia-reperfusion injury by human IL-10 gene transduction in rats. Methods Ad-hIL10-EGFP (1. 0 ? 109 plaque forming units/ml) was administered into SD rats by intravenous injection 72 hours before hepatic ischemia-reperfusion injury was induced. Liver function were tested and HE pathology was observed. The expression of hIL-10 was studied with ELISA or immunohistochemical method, the expression of EGFP was observed in frozen sections under the fluoroscopy. The apoptosis of hepatocytes was observed with Tunel's assay. Results Compared with control rats, the expression of EGFP and hIL-10 was observed, serum hIL-10 level was (815.74 ? 284. 76) ng/ml, liver function of treatment rats were improved, the paraffin sections showed that the hepatocytes were not significantly swelling and liver pathology ameliorated, the number of apoptosis cells decreased (P
