Immune mechanisms in the pathogenesis of periventricular leukomalacia in neonatal rats caused by lipopolysaccharide
- VernacularTitle:内毒素致幼鼠脑室周围白质软化实验研究
- Author:
Dapeng JIANG
;
Fuyou HAN
;
You YU
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Cerebral Palsy;
Periventricular, leukomalacia
- From:
Chinese Journal of Perinatal Medicine
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the animal model of periventricular leukomalacia (PVL) caused by lipopolysaccharide (LPS) and investigate the immune mechanisms in the pathogenesis of cerebral palsy due to intrauterine infection. Methods Twenty-six mature pregnant Wistar rats were divided into experiment(n= 15)and control groups(n =11). LPS(0. 3 mg/kg) and distilled water were injected on 15 days of gestation to two groups respectively intraperitoneally. One hundred and twenty-eight neonatal rats of experiment group and 105 in the control group were obtained till the gestation day of 21. The level of IL-2, TNF-? and histology changes in neonatal rats' brain were observed by HE staining and RIA, water content in brain tissues were compared. Results IL-2[(3. 56?0. 450) ng/g] and TNF-? concentrations [(8. 23?0. 47)ng/g] in neonatal rats' brain of the experiment group were significantly higher than those in the control group [(0. 78?0. 2)ng/g and (5. 23?0. 32)ng/g, P
