Effects of selenium enriched Spirulina platensis on antioxidation and regeneration of rat hepatocytes after hepatectomy
- VernacularTitle:硒螺旋藻对大鼠肝叶切除术后肝细胞再生和抗氧化能力的影响
- Author:
Feng HUANG
;
Zhi HUANG
;
Fang YANG
;
Wenjie ZHENG
;
Xiaolin ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatectomy;
Liver Regeneration;
Se-SP
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of selenium enriched Spirulina platensis(Se-SP) on(antioxidation) and regeneration of rat hepatocytes after partial hepatectomy.Methods Sixty-seven percent(hepatectomy) was performed in rats after high dose(H) and low dose(L) Se-SP was given via gastric tube for 7 days.Then selenium(Se),glutathione peroxidase(GPx),superoxide dismutase(SOD) and(malondiaoldehyde)(MDA) in hepatocytes were determined.The results were compared with normal saline(giving) via gastric tube before hepatectomy(C) and sham operation(S) groups.Hepatocytes were obtained(before) operation and 24h after operation,and the expression index of proliferating cell nuclear antigen((PCNA)) in hepatocytes was detected by immunohistochemistry,and the DNA damage of hepatocytes induced by peroxide was analyzed by comet assay.Results After partial hepatectomy,Se content,GPx and SOD(activity),and PCNA expression index were obviously higher(P
