Effect of survivin antisense oligodeoxynucleotide on intimal hyperplasia of vein graft
- VernacularTitle:转染survivin反义寡脱氧核苷酸对移植血管内膜增生的影响
- Author:
Jun YANG
;
Xinhua HU
;
Chengwei LIU
;
Zhishen ZHANG
;
Qiang ZHANG
- Publication Type:Journal Article
- Keywords:
Oligodeoxynucleotides,Ahtisense/pharm;
Endothelium,Vascular/durg eff;
Vein Grafting
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of survivin antisense oligodeoxynucleotides(ASODN) on(intimal) hyperplasia(IH) in vein graft in rats.Methods Autogenous vein graft model was established in 60 Wistar rats by transplanting the interior jugular vein to common jugular artery using microsurgical technique.The rats were divided into 5 groups according to different processing methods,including survivin ASODN 50?g and 200?g,scramble ODN 200?g,control group and lipoectin+pluronic group.Vein graft samples were harvested at 1 or 2 weeks after surgery and histomorphologic methods were used to compare the degree of IH.Survivin mRNA was measured by RT-PCR.Western blotting and immunohistochemistry methods also were used to detect the expression of survivin and PCNA.Apoptosis of VSMC was dectected by TUNEL.Results IH was evident within 1 to 2 weeks after vein grafting,but was markedly inhibited by 50?g of survivin ASODN(P
