Effect of adrenomedullin on tissue factors and TFPI expression in HUVECs treated with oxLDL
- VernacularTitle:肾上腺髓质素对oxLDL诱导的HUVECs组织因子及其抑制物表达的干预作用
- Author:
Wei LIU
;
Ziqiang ZHU
;
Shuyu ZU
;
Guangjin ZHU
- Publication Type:Journal Article
- Keywords:
Adrenomedullin;
Lipoprotein, LDL;
Arteriosclerosis;
Thromboplastin
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the role of adrenomedullin (AM) in tissue factor (TF) and TFPI expression in HUVECs stimulated with oxLDL and to investigate its possible signal transduction pathway. METHODS: Using chromogenic assay and RT-PCR technique, TF and TFPI protein activity and mRNA level in cultured HUVECs were observed. The signal transduction pathway of AM action was further analyzed by applying Rp-cAMP (cAMP antagonist), PD098059 (MAPK inhibitor) and H7 (PKC inhibitor). RESULTS: AM inhibited TF protein activity and mRNA expression in HUVECs treated with oxLDL in a concentration-dependent fashion; AM alone increased TFPI protein activity and mRNA expression in concentration-dependent and time-dependent manner. Moreover, AM reversed the decrease in TFPI protein activity and mRNA level caused by oxLDL; AM-induced TFPI expression was inhibited by cAMP and MAPK inhibitors. CONCLUSION: AM can reverse the effects of oxLDL on TF and TFPI expression in HUVECs, which can help improve the state of blood coagulation in atherosclerosis and delay development of atherosclerosis.
