Identification of down-regulated genes in cardiac fibroblasts stimulated by angiotensin Ⅱ
- VernacularTitle:心肌成纤维细胞在血管紧张素Ⅱ刺激下表达下调基因的分离和鉴定
- Author:
Xinfeng WANG
;
Guangdao GAO
;
Jian LIU
;
Yuanxi LIN
;
Rong GUO
;
Yonglie CHU
;
Xingli SU
;
Fengchan HAN
;
Wenhong ZHANG
;
Yujie BAI
- Publication Type:Journal Article
- Keywords:
AngiotensinⅡ;
Myocardium;
Fibroblasts;
Suppression subtractive hybridization;
Gene expression
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To identify the down-regulated genes in adult rat cardiac fibroblasts (CF) stimulated with angiotensin Ⅱ (AngⅡ). METHODS: Suppression subtractive hybridization (SSH) was performed between the CF stimulated by AngⅡ (driver) and unstimulated CF (tester) to generate subtractive cDNA library. The library was screened with dot blots hybridization to further verify the differentially expressed cDNA clones. Partial positive clones were sequenced and BLAST analyzed. RESULTS: Seventeen down-regulated genes related to intracellular signal transduction, transcriptional repression, deposition of fibrous matrix and cellular cytoskeletal rearrangement, and 4 new expression sequence tags (EST) were acquired. CONCLUSION: SSH is a powerful technique with high sensitivity for the detection and clone of down-regulated genes expressed in CF induced by AngⅡ, which is helpful to clarify the mechanism of cardiac remodeling.
