Effects of lipoteichoic acid-induced delayed preconditioning on cardioplegic arrest/reperfusion injury in donor rat heart
- VernacularTitle:脂质胞壁酸延迟预适应对大鼠供心停搏/复灌性损伤的影响
- Author:
Shiyu MA
;
Jizhou XIANG
;
Jiliang WU
;
Yexin MA
- Publication Type:Journal Article
- Keywords:
Lipoteichoic acid;
Preconditioning;
Reperfusion injury;
Organ preservation
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the potential effects of lipoteichoic acid (LTA)-induced delayed preconditioning (PC) on cardioplegic arrest/reperfusion injury in donor rat heart. METHODS: The rats were pretreated with LTA (1 mg/kg, ip) 24 h before the experiment, and the isolated hearts were subjected to arrested by cardioplegic solution and stored in Eurocollin's solution for 4 h by the Langendorff method, and to evaluate the changes of cardiac function at the reperfusion for 30 min and 60 min, to measure the amounts of MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH) and total nitric oxide (NO) oxidation products in the coronary effluent, and to detect myocardial apoptosis on tissue samples of left ventricle at the end of reperfusion by TUNEL staining. RESULTS: Pretreated with LTA significantly improved the recovery of cardiac function with a significant increase in coronary flow (CF), left ventricular developed pressure (LVDP), maximal rate of left ventricular developed pressure (+dp/dt_ max), and minimal rate of left ventricular decline pressure (-dp/dt_ max) at 30 min and 60 min of reperfusion (all P
