Preconditioning effect of nitrate medicine on different parts of the heart and its signaling pathway
- VernacularTitle:各部位心肌细胞对硝酸酯类药物预适应能力的实验研究
- Author:
Cheng WANG
;
Hongwei LI
;
Ailing LI
;
Jing ZHANG
;
Ruijuan XIU
- Publication Type:Journal Article
- Keywords:
Cardiomyocytes;
Nitric oxide;
Anoxia;
Preconditioning
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the preconditioning effect of nitrate medicine on different parts of the heart and its signaling pathway. METHODS: The cells from different parts of the heart (atrium, left ventricle, right ventricle, apex and the whole heart) were isolated and cultured. The cultured cells were treated with hypoxia and reperfusion, hypoxia preconditioning, 1 mmol/L L-arginine preconditioning and 5 mmol/L L-arginine preconditioning, respectively. The cell necrosis rate, cell apoptosis rate, LDH concentration in the medium, the [Ca 2+]i and PKC activity in the cells were determined. RESULTS: The result show that hypoxia and reperfusion increased the necrosis rate and apoptosis rate, enhanced the concentration of LDH in the medium and the [Ca 2+]i overload in the cells (P0.05). After hypoxia preconditioning and L-arginine preconditioning, these indexes decreased (P
