Effects of crocetin on the apoptosis and the changes of its related regulating proteins caspase-3 and Bcl-2 induced by H_2O_2 in myocardial cells
- VernacularTitle:西红花酸对H_2O_2诱发心肌细胞凋亡及相关调控蛋白caspase-3、Bcl-2表达改变的影响
- Author:
Weiping YU
;
Guanglin XU
;
Chengxing SHEN
;
Zhiyu QIAN
- Publication Type:Journal Article
- Keywords:
Crocetin;
Hydrogen peroxide;
Myocardium;
Apoptosis;
Caspase-3;
Protein Bcl-2
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of crocetin on the apoptosis and the changes of its related regulating proteins caspase-3 and Bcl-2 expression induced by hydrogen peroxide (H_2O_2) in cultured cardiomyocytes. METHODS: Changes of cellular morphology were detected under microscope. Apoptosis rates of the cells were analyzed by PI staining with flow cytometry. Expressions of caspase-3 and Bcl-2 proteins in the cells were determined by immunofluorescence with flow cytometry. RESULTS: In the concentrations used, more severe morphological changes with higher apoptosis rate of the cultured myocardial cells were seen in each H_2O_2 group than that in control group. When treated with 1?10 -4 mol?L -1 H_2O_2, the caspase-3 was increased and Bcl-2 protein decreased remarkably in the cells. But each dosage of crocetin, especially the highest one (5?10 -5 mol?L -1, P
