Effect of MIF antisense oligonucleotides on expression of MIF in macrophages
- VernacularTitle:MIF反义寡核苷酸对巨噬细胞表达MIF的影响
- Author:
Guangran LI
;
Weiying CHEN
;
Xueqing YU
;
Xiaoyan LI
;
Xiao YANG
- Publication Type:Journal Article
- Keywords:
Macrophages;
Macrophage migration-inhibitory factors;
Oligonucleotides, antisense
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of antisense oligonucleotides on expression of macrophage migration inhibitory factor (MIF) in macrophages. METHODS: MIF phosphorothioate oligonucleotides was designed and synthesized. The phosphorothioate antisense, sense and missense oligonucleotides of mouse MIF was transfected into macrophages, separately. After that, macrophages were incubated with LPS. Cell culture medium was collected for MIF protein detection by EIA. Cellular RNA was extracted and the expression of MIF mRNA was examined by RT-PCR analysis. RESULTS: LPS stimulation resulted in a specific time-dependent expression of MIF derived from macrophages. MIF mRNA and MIF protein level increased at 6 h and reached a plateau at 9-12 h after LPS stimulation. The macrophages treated with antisense oligonucleotides showed a significant decrease in MIF mRNA and MIF protein after LPS stimulation than those with LPS stimulation only and LPS plus sense or missense oligonucleotides (P
