Peripheral blood mononuclear cells activated by graded zirconia-hydroxyapatite composite in vitro
- VernacularTitle:二氧化锆梯度复合羟基磷灰石体外激活末梢血单个核细胞的实验研究
- Author:
Renfu QUAN
;
Disheng YANG
;
Xudong MIAO
;
Wei LI
;
Xiaochun WU
;
Hongbin WANG
- Publication Type:Journal Article
- Keywords:
Zirconium;
Hydroxyapatites;
Monocytes;
Tumor necrosis factor;
Interleukin-6
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the immunogenicity of a novel orthopedics materials (graded zirconia-hydroxyapatite composite) in vitro by using peripheral blood mononuclear cells (PBMCs) from healthy young people, and simple zirconia-hydroxyapatite composited material was used as control materials. METHODS: Proliferation of PBMCs cultured in different liquid after 5 days was measured by MTT methods. ELISA was used to detect TNF-? and IL-6 concentration in the supernatant of PBMCs cultured in the extracts after 24 hours. Flow cytometery was used to measure CD69 and CD25 in activated PBMCs cultured in the extracts of the two kinds of materials after 24 hours. RESULTS: The proliferation rate of the simple composite group was significantly lower than that in negative group (P
