Immune escape of human lung carcinoma cell lines mediated by Fas/FasL pathway
- VernacularTitle:Fas/FasL途径介导的人肺癌细胞免疫逃逸
- Author:
Qing XIANG
;
Bo XU
;
Mei XU
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
T-lymphocytes;
Fas/FasL;
Jurkat cells
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of Fas/FasL system in human lung carcinoma cell lines (A549, EBC-1, LCSC) and T-lymphocytes (Jurkat), and to search the possibilities of immune escape and counterattack mediated by Fas/FasL pathway in human lung carcinoma cells. METHODS: The protein and mRNA expression of Fas and FasL were detected by FACScan, RT-PCR, respectively. Cell apoptosis was assessed by fluorescent staining. Cell growth was determined by a trypan blue exclusion assay. RESULTS: Fas and FasL were expressed in 3 human lung carcinoma cell lines and T-cells, Jurkat. The lung carcinoma cells inhibited the growth (P
