Polymorphism within the interleukin-10 receptor cDNA gene in patients with systemic lupus erythematosus
- VernacularTitle:系统性红斑狼疮患者白介素10受体cDNA基因多态性研究
- Author:
Xu YAO
;
Yumei LI
;
Zijia HU
;
Wenzhong LI
;
Zhiqiang CHEN
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus, systemic;
Receptors, interleukin;
Polymorphism, single-stranded conformational
- From:
Chinese Journal of Dermatology
1995;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the association between polymorphism within the interleukin-10 receptor cDNA gene (IL-10R) and Chinese patients with systemic lupus erythematosus (SLE). Methods The IL-10R genotypes of 94 SLE patients and 80 healthy subjects were examined by reverse transcription-polymerase chain reaction-single strand conformation polymorphism method (RT-PCR-SSCP), RT-PCR-restriction fragment length polymorphism (RFLP) and DNA sequencing. Results There were significant differences in the IL-10R2 genotype frequencies of these two groups. The IL-10R2 G520/G520 genotype increased the risk of developing SLE (OR = 0.515, 95% CI 0.414-0.579, P = 0.004) and individuals who had G520/A520 genotype also had a higher susceptibility to SLE (OR = 1.968, 95% CI 0.981-3.949, P = 0.055). There was no significant association between SLE and IL-10R1 genotypes. The risk of developing SLE was detected in the individuals who had the combination of IL-10R1 G241/G241 and IL-10R2 G520/G520 (OR = 0.515, 95% CI 0.444-0.597, P = 0.004). Conclusion The IL-10R2 genotypes of G520/G520 and G520/A520 as well as the combination of genotypes IL-10R1G241/G241 and IL-10R2 G520/G520 may increase the susceptibility to SLE in Chinese people.
