The effect of RNA interference on the expression of CTGF in skin fibroblasts of systemic sclerosis
- VernacularTitle:RNA干扰对系统性硬化病皮肤成纤维细胞结缔组织生长因子表达的影响
- Author:
Rong XIAO
;
Jingying LUO
;
Fuyou LIU
;
Ying ZHOU
;
Yaping LI
;
Yixin ZHOU
;
Yuwen SU
;
Haiquan WEN
;
Xinjie YANG
- Publication Type:Journal Article
- Keywords:
RNA, small interfering;
Scleroderma, systemic;
Fibroblast;
Connective tissue growth factor
- From:
Chinese Journal of Dermatology
1995;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of RNA interference on the expression of CTGF in skin fibroblasts of systemic sclerosis(SS). Methods Four CTGF specific siRNAs and a negative control siRNA were designed and then synthesized by in vitro transcription. siRNAs labeled with carboxyfluorescein-6-succimidyl ester (FAM) were transiently transfected into SS skin fibroblasts. Forty-eight hours after the fibroblasts were treated with siRNAs, the mRNA and protein expression of CTGF was detected by semiquantitative RT-PCR and Western blot analysis, respectively. Results The mRNA and protein expression of CTGF in fibroblasts was significantly down-regulated by 4 and 3 CTGF specific siRNAs (both P
