Study on protection of Bcl-2 on islet cells cultured in immunosuppressive drugs
- VernacularTitle:Bcl-2对免疫抑制剂培养中胰岛细胞的保护作用
- Author:
Haibing JU
;
Zizheng SHU
;
Dengming HU
;
Huiying YING
;
Feifei SHEN
;
Ziying ZHU
- Publication Type:Journal Article
- Keywords:
Genes,bcl-2;
Islets of langerhans;
Tacrolimus binding proteins;
Immunosuppressive agents
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the toxic effect of immunosuppressive drugs on apoptosis of rat pancreatic islet cells in vitro and the protective action of Bcl-2.Methods Islet cells expressing Bcl-2 and the control islet cells were cultured at different concentrations of tacrolimus and the apoptosis rate of islet cells and insulin accumulation in the culture medium were detected after 48h.Results Low and high concentrations of tacrolimus induced the apoptosis of islet cells and decreased insulin secretion.The Bcl-2 inhibited the apoptosis of islet cells induced by tacrolimus and improved the insulin secretion.Conclusion Tacrolimus may directly damage to isolated rat islet cells and the expression of Bcl-2 can protect the cells from the damage of immunosuppressive drugs.
