Albumin stimulates osteopontin mRNA expression in renal tubular epithelial cells through p38MAPK activation
- VernacularTitle:p38信号通路对白蛋白诱导肾小管上皮细胞骨调素mRNA的表达
- Author:
Hua SHUI
;
Qun WANG
- Publication Type:Journal Article
- Keywords:
p38 mitogen-activated protein kinase;
Albumins;
Kidney tubules;
Osteocalcin
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roll of p38MAPK on the expression of osteopontin(OPN) mRNA in cultured renal tubular epithelial cells stimulated by albumin.Methods The normal renal tubular epithelial cells(NRK-52E) were incubated in the presence of albumin(30mg/ml) with or without SB203580,an inhibitor of p38MAPK.Reverse transcriptase polymerase chain reaction(RT-PCR) was performed to determine the expression of OPN mRNA and the p38MAPK phosphorylation was evaluated by Western blot analysis.Results Albumin stimulated the phosphorylation of p38MAPK in a timedependent manner,and up-regulated the expression of OPN mRNA.SB203580 could inhibit the expression of OPN mRNA.Conclusion The p38MAPK signal pathway plays an important roll in OPN mRNA expression stimulated by Albumin.
