Establishment of two-dimensional gel electrophoresis technical platform for the blood serum proteome research in patients with end stage renal disease
- VernacularTitle:终末期肾病患者血清蛋白质组二维电泳图像与正常血清蛋白图谱比较研究
- Author:
Jianqing WANG
;
Yong DAI
;
Anguo DENG
;
Jianjun LIU
- Publication Type:Journal Article
- Keywords:
Kidney failure;
Proteome;
Electrophoresis,gel,tow dimensional
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and optimize the two-dimensional gel electrophoresis technical platform for the blood serum proteome research in patients with end stage renal disease(ESRD).Methods Immobiline pH gradients isoelectric focusing was used as the first dimensional gel electrophoresis and the vertical SDS-PAGE was used as the second dimensional gel electrophoresis(2-DE).The 4 differentially expressed protein spots were identified by mass spectrometry.Results Satisfactory 2-DE maps of ESRD patients serum protein were obtained and there were some differentially protein spots between the 2-DE maps of ESRD patients and normal controls.Conclusions The 2-DE technology for the serum proteome of ESRD patients is set up.
