Effects of dendritic cells pulsed by hepatitis B surface antigen on the functions of cytokine-induced killer cells
- VernacularTitle:HBsAg脉冲的树突状细胞对CIK细胞功能的影响
- Author:
Shuren LIU
;
Yang ZHANG
;
Qing XIE
;
Zhuoliang LI
;
Zhouyao YU
;
Xiangping KONG
- Publication Type:Journal Article
- Keywords:
Hepatitis B;
Hepatitis B surface antigens;
Dendritic cells;
Killer cells
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of dendritic cells(DCs) pulsed by hepatitis B surface antigen(HbsAg) on the proliferation and function of cytokine-induced killer(CIK) cells.Methods The peripheral blood mononuclear cells(PBMCs) were isolated by the conventional method,pulsed by HbsAg,and added into the CIK cells.The ~3H-TdR incorporation was used to determine the proliferation of CIK cells and lactate dehydrogenase(LDH) release was used to measure the specific killer activity of CIK cells induced by HBsAg-pulsed DCs.Results The HBsAg-pulsed DCs could induce the proliferation of CIK cells and strengthen the killer activity of CIK cells induced by HBsAg-pulsed DCs(P
