Regulation of gypenosides on TGF-?/Smad signaling pathway and expression of connective tissue growth factor in unilateral ureteral obstruction rats with renal tubulointerstitial fibrosis
- VernacularTitle:绞股蓝总皂甙对梗阻性肾病大鼠TGF-?/Smad信号通路及CTGF表达的影响
- Author:
Yong ZHANG
;
Guohua DING
;
Jiee ZHANG
;
Houqin XIAO
;
Pingyong WU
;
Faguo LE
- Publication Type:Journal Article
- Keywords:
GYPENOSIDE;
Nephrosis;
Intercellular signaling peptides and proteins;
Transforming growth factor beta
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression transforming growth factor?1(TGF-?1),Smad7 and connective tissue growth factor(CTGF) in rats with renal tubulointerstitial fibrosis and the effects of Gypenosides(GPs) treatment on expression of TGF-?,Smad7 and CTGF.Methods Unilateral ureteral Obstruction(UUO) rat animal models were used and the rats were divided into GPs group(Gypenosides 200 mg?Kg~(-1)?d~(-1) by gastric gavage from 3d before the obstruction to 14 days after the induction),model group and sham operated group.The expression of TGF-?1,Smad7 and CTGF in the obstructed kidneys was assessed by RT-PCR,immunohistochemistry and HE staining methods after 3,7,and 14d respectively.The degree of tubulointerstitial damage was scored by Masson staining methods.Results Compared with the sham operated group,the expressions of TGF-?1,CTGF mRNA and protein were markedly increased in UUO group,and the expression of Smad7 mRNA and protein was markedly decreased(all P
